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Sample preparation solutions

Dilute and shoot (D&S) and liquid-liquid extractions (LLE) are fast and easy sample preparation methods for low complexity matrices and high abundance analytes in liquid.

Several extraction methods can be combined on one Freedom EVO® platform, for maximum flexibility and to future-proof your investment.

Tecan’s Fluent® platform provides optimal clinical MS sample preparation for intra-cellular analytes.




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Tab 01 / Overview
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Find out which smart sample prep solution best suits your application. Click here!

D&S and LLE workflows are employed in many laboratories for low complexity analyses and abundant analytes.

Automating LLE is especially challenging because of the phase separation steps.

Tecan offers a range of automated solutions that can be adapted to meet your needs, from pipetting and sample tracking to full automation with one or more separation techniques.

The platforms are upgradeable, allowing you to increase the level of automation in line with your investment plans.




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Tab 02 / Clinical MS sample preparation​
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Automate whole blood sample preparation for LC-MS on the
Fluent® Mix and Pierce Workstation

Whole blood screening is performed regularly for patients taking drugs that are absorbed into blood cells, such as immunosuppressants taken after organ transplants.

Automation of whole blood liquid handling eliminates tedious and contamination-prone tube decapping and capping steps, provides homogenous sample mixing, and delivers excellent pipetting performance, to standardize results in the clinical arena. ​

Fluent 480 with Flexible Channel Arm™ (FCA) using four Tecan Piercing Tips™ and four disposable tip adapters
Fluent Mix and Pierce Workstation Tube Rotator™ with capacity for 120 primary sample tubes
Fluent ID™ barcode reader
Integrated BioShake® 3000 elm DWP shaker (Qinstruments GmbH)
Trough carriers for reagents and tube runner with calibration samples and internal standard
Disposable tip boxes

The Fluent Mix and Pierce Workstation is a product configuration for sample distribution based on the Fluent Automation Workstation, a fully automated laboratory liquid handling platform for general purpose use. It is not linked to a particular assay, and Tecan is not responsible for any assay claims.




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Tab 03 / MS based proteomics​
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Automation for mass spectrometry-based proteomics workflows​

Mass spectrometry (MS)-based proteomics is the most comprehensive approach for quantitative profiling of proteins, their interactions and modifications. The main challenge is the biochemistry expertise required for sample preparation, as well as experience with MS instrumentation and the subsequent computational biology data analysis.

To ease this pain point. Tecan has established various automated methods for sample preparation processes using its NBE columns and commercially available kits and consumables from PreOmics and ProtiFi to convert proteins into peptides. ​

Sample preparation for MS-based proteomics: overview

Application notes and Poster

This application note describes high throughput automation of the SP3-iST protocol on a Freedom EVO workstation, with automated peptide clean-up using the Resolvex A200 positive pressure processor.

This automated workflow enables the processing of up to 96 samples reducing overall hands-on time to less than one h

Mass spectrometry (MS) has emerged as the leading technology for the identification and quantification of protein populations in numerous sample types.

This technology has become much faster and increasingly sensitive over the last decade, broadening the scope of the research questions that it can address.

Process speed and efficiency have created a demand for simplified sample preparation approaches, leading to the development of off-the-shelf sample preparation kits, such as PreOmics’ iST technology.

This application note describes the full automation of the TMTpro 16plex labeling protocol using the Freedom EVO® liquid handling platform.

This workflow allows up to six TMTpro 16plex experiments to be processed in one run, increasing throughput and reproducibility, and eliminating the potential for operator errors.

This application note describes the automation of the Waters AccQ-Tag Ultra Derivatization Kit on a Fluent workstation.

Starting with hydrolyzed samples, this automated workflow enables the processing of up to 89 samples, together with a 7-point calibration curve, with minimal user intervention. Following this kit’s protocol, it is possible to prepare a full 96-well plate in around 1 hour.




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Tab 04 / Liquid Liquid Extraction
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Freedom EVO® LLE/protein precipitation workstation

LLE is a sample extraction method that separates analytes based on their relative solubility in two different immiscible liquids, and is widely employed by clinical, medicinal chemistry and food labs.

Despite its popularity, the technique is very labor intensive and poses a number of challenges; variable extraction yields, high solvent consumption and operator health risks due to exposure to hazardous organic solvents. Automation can reduce these risks and lead to more reproducible results.

Freedom EVO® 100 with four-channel Air LiHa™
Trough carriers for reagents
Tube racks for samples
PosID™ barcode reader
Te-Shake™ module
Hettich Rotanta 460 centrifuge, temperature controlled
Freedom EVOware® standard, TouchTools™ LCMS Data Converter




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Tab 05 / Dilute and Shoot
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Workstation

Toxicology and hospital laboratories performing urinary drug screening routinely use dilute and shoot for simple sample preparation. In some cases, a hydrolysis reaction is also performed to release the analyte from any moiety it is coupled to.

Automation offers a cost-effective solution for dilute and shoot workflows, helping to reduce repeat tests, increase productivity and enhance data quality. The challenge is to reduce ion suppression, matrix interference and increased maintenance of LC-MS equipment.

Freedom EVO® 100 Air with 4-channel liquid handling
Trough carriers for reagents
Tube racks for samples
PosID
Te-Shake
Freedom EVOware® standard, TouchTools™ LCMS Data converter
Related products
Freedom EVO series

Unlimited options, individual solutions - one reliable platform

Fluent series

Simplify your lab with industry-leading application focused automation

 
Positive pressure workstations

The Resolvex smart extraction consumables have been optimized to work with our suite of Resolvex positive pressure SPE workstations.

Smart extraction consumables

Tecan's offers an extensive range of smart consumables that can be easily integrated.

 

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